Motility can be checked by microscopy using the hanging drop technique. Motility media includes SIM agar (see Indole test) and
TTC motility media which contains a tetrazolium salt which changes color where bacterial growth is located. In both cases growth
along the inoculation line only indicates non-motile organisms while diffuse or confluent growth through the tube indicates motility.
Hanging drop setup for observing motility under the microscope