LAB PROCEDURES MANUAL

by Jackie Reynolds
Richland College

revised Fall 2005

This online lab manual is to be used in conjunction with the PHOTO ATLAS FOR THE MICROBIOLOGY LABORATORY by Leboffe and Pierce.  Although I have included some pictures, drawing, or photos to accompany some of the exercises, I have purposely tried to keep the graphics down to a minimum to save paper and, frankly, the pictures in the PHOTO ATLAS are much better than I could make anyway. You can download and print all of the exercises for the entire semester.  In the table of contents, I have listed the pages from the PHOTO ATLAS that correlate with these lab exercises.  There is an online GLOSSARY, linked to terms within the text of the lab exercises.

Please read the procedures before coming to lab so you have an idea of what will be done that day.
 
 

Categories of Exercises	Name of Exercise (see Photo Atlas)
Introduction	1. Mock Epidemic
Growth of Microbes	2. Media Preparation   3. Aseptic Transfers  4. Dilution Techniques & Pipetting 5. Dilution Worksheet & Problems   6. Unknown Bacteria from a Sponge  7. O2 Requirements & Anaerobes 8. Cultures from Ear, Nose, & Throat  9.  Bacterial Colony Morphology 10. Kirby-Bauer (Antibiotic Sensitivity) 11. Bacterial Numbers   12. Antimicrobials: Disinfectants,  Antiseptics, and Spices  13. Oral Biofilms
Microscopy	14. Use of the   Microscope  15. Bacterial Smears (with microscope) 16. Hanging Drop Wet Mount   17. Simple Stains 18. Gram Stain  19. Spore Stain  20. Acid-fast stain  21. Flagella Stain 22. Capsule Stain
UNKNOWNS	Gram + and G - unknowns  Media List for unknown identification  Miscellaneous media procedures Unknown ID sheets to turn in (.pdf)
Biochemical Tests	Biochemical test table (.pdf) for summary of test reactions 23. EMB  (with throat)  24. CNA  (with throat) 25. OF glucose   26. Catalase Test   27. Oxidase Test   28. Starch hydrolysis  29. Lipid Hydrolysis   30. Casein Hydrolysis   31. Coagulase Test   32. Mannitol Salt Tests   33. Deoxyribonuclease Test   34. Litmus Milk   35. Carbohydrate Use   36. Bile Esculin  37.   IMViC tests:         Indole Production (SIM)         Methyl Red & Voges-Proskauer        Citrate Test  38. Motility Agar  39. Gelatin Hydrolysis 40. Triple Sugar Iron Agar  41. Decarboxylation of Amino Acids  42. Deamination of Amino Acids  43. Nitrate Reduction   44. Urea Hydrolysis  45. Beta-Galactosidase Test (ONPG)   46. API-20E multitest strip
Food & Water Microbiology	47. Water Analysis  48. Meade   49. Yogurt
Eucaryotic Organisms & Viruses	50. Kombucha  51. Identifying Fungi   52. Protozoa    53. Bacteriophages   54. Helminths   55. Algae
Biotechnology	56. DNA Restriction & Electrophoresis   57. Bacterial Transformation  58. Bacterial Phenotypes
Immunity	59. Antigen-Antibody Tests

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